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Last Posted: Jan 26, 2023
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Use of Wastewater for Mpox Outbreak Surveillance in California.
Wolfe Marlene K et al. The New England journal of medicine 2023 1

As part of the response to the spread of MPXV infection, we adapted and deployed polymerase-chain-reaction assays targeting MPXV genomic DNA5 as part of our ongoing wastewater surveillance program. Testing was implemented within a month after the first identified case of MPXV infection in the United States, and the results were used in real time for the public health response in California.

Development of an amplicon-based sequencing approach in response to the global emergence of human monkeypox virus
NFG Chen et al, MEDRXIV, January 13, 2023

Highly multiplexed amplicon-based sequencing (PrimalSeq) was initially developed for sequencing of Zika virus, and later adapted as the main sequencing approach for SARS-CoV-2. Here, we used PrimalScheme to develop a primer scheme for human monkeypox virus that can be used with many sequencing and bioinformatics pipelines implemented in public health laboratories during the COVID-19 pandemic. We sequenced clinical samples that tested presumptive positive for human monkeypox virus with amplicon-based and metagenomic sequencing approaches.

Detection of Monkeypox Virus DNA in Airport Wastewater, Rome, Italy.
La Rosa Giuseppina et al. Emerging infectious diseases 2022 12 (1)

Wastewater surveillance for SARS-CoV-2 was adapted for MPX virus detection from the wastewater treatment plant of an international airport with >3M passengers monthly (Rome, Italy, May–Aug 2022). Real-time and nested PCR assays were modified to detect mpox DNA in 24hr composite wastewater samples collected 2x/week. Among 20 samples, 3 had detectable MPXV. Average quantification cycle (Cq) ranged from 38.37–40.18, indicating relatively low DNA concentrations. Consensus sequences found 100% similarity with MPXV strains available in GenBank.

A Multi-Laboratory Evaluation of Commercial Monkeypox Molecular Tests
O Erster et al, MEDRXIV, November 29, 2022

In this report, we describe the first national scale multi-laboratory evaluation of commercial quantitative PCR kits for detection of Monkeypox virus (MPXV) DNA. The objective of this study was to assess the performance of two kits by different diagnostic laboratories across Israel. A panel of 10 standardized samples was tested simultaneously using the Novaplex (15 laboratories) and Bio-Speedy (seven laboratories) kits. An in-house assay based on previously published tests was used as reference. Comparison of the results showed high intra-assay consistency between laboratories, with small variations for most samples.


Disclaimer: Articles listed in the Public Health Genomics and Precision Health Knowledge Base are selected by the CDC Office of Public Health Genomics to provide current awareness of the literature and news. Inclusion in the update does not necessarily represent the views of the Centers for Disease Control and Prevention nor does it imply endorsement of the article's methods or findings. CDC and DHHS assume no responsibility for the factual accuracy of the items presented. The selection, omission, or content of items does not imply any endorsement or other position taken by CDC or DHHS. Opinion, findings and conclusions expressed by the original authors of items included in the update, or persons quoted therein, are strictly their own and are in no way meant to represent the opinion or views of CDC or DHHS. References to publications, news sources, and non-CDC Websites are provided solely for informational purposes and do not imply endorsement by CDC or DHHS.

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