Use of Wastewater for Mpox Outbreak Surveillance in California.
Wolfe Marlene K et al. The New England journal of medicine 2023 1
As part of the response to the spread of MPXV infection, we adapted and deployed polymerase-chain-reaction assays targeting MPXV genomic DNA5 as part of our ongoing wastewater surveillance program. Testing was implemented within a month after the first identified case of MPXV infection in the United States, and the results were used in real time for the public health response in California.
Development of an amplicon-based sequencing approach in response to the global emergence of human monkeypox virus
NFG Chen et al, MEDRXIV, January 13, 2023
Highly multiplexed amplicon-based sequencing (PrimalSeq) was initially developed for sequencing of Zika virus, and later adapted as the main sequencing approach for SARS-CoV-2. Here, we used PrimalScheme to develop a primer scheme for human monkeypox virus that can be used with many sequencing and bioinformatics pipelines implemented in public health laboratories during the COVID-19 pandemic. We sequenced clinical samples that tested presumptive positive for human monkeypox virus with amplicon-based and metagenomic sequencing approaches.
Detection of Monkeypox Virus DNA in Airport Wastewater, Rome, Italy.
La Rosa Giuseppina et al. Emerging infectious diseases 2022 12 (1)
Wastewater surveillance for SARS-CoV-2 was adapted for MPX virus detection from the wastewater treatment plant of an international airport with >3M passengers monthly (Rome, Italy, May–Aug 2022). Real-time and nested PCR assays were modified to detect mpox DNA in 24hr composite wastewater samples collected 2x/week. Among 20 samples, 3 had detectable MPXV. Average quantification cycle (Cq) ranged from 38.37–40.18, indicating relatively low DNA concentrations. Consensus sequences found 100% similarity with MPXV strains available in GenBank.
A Multi-Laboratory Evaluation of Commercial Monkeypox Molecular Tests
O Erster et al, MEDRXIV, November 29, 2022
In this report, we describe the first national scale multi-laboratory evaluation of commercial quantitative PCR kits for detection of Monkeypox virus (MPXV) DNA. The objective of this study was to assess the performance of two kits by different diagnostic laboratories across Israel. A panel of 10 standardized samples was tested simultaneously using the Novaplex (15 laboratories) and Bio-Speedy (seven laboratories) kits. An in-house assay based on previously published tests was used as reference. Comparison of the results showed high intra-assay consistency between laboratories, with small variations for most samples.