RC-PCR WGS applicability for outbreak analysis was tested on 6 predefined clusters containing samples of healthcare workers and patients. RC-PCR resulted in WGS data for 146 samples. 3 out of 6 suspected clusters were fully confirmed, while in other clusters four healthcare workers were not associated. A previously unknown chain of transmission was confirmed.

We systematically explored genetic polymorphisms associated with the clinical outcomes in SARS-CoV infection in humans. literature search comprised available English papers published in PubMed/Medline and SCOPUS databases following the PRISMA-P guidelines and PICO/AXIS criteria. 29 polymorphisms in 21 genes were identified as associated with SARS-CoV susceptibility/resistance, disease severity, and clinical outcomes predominantly in Asian populations.

Employing phylogenetic methods on Danish genome sequences of SARS-CoV-2, we exemplify how genetic data can be used to trace the introduction of a virus to a country. Our analysis supports the hypothesis that the virus was brought to Denmark by skiers returning from Ischgl. On the other hand, we identify transmission routes which suggest that Denmark was part of a network of countries among which the virus was being transmitted.

Here we present genomic surveillance data on 102 imported cases, which account for 17.2% of the total cases in Beijing. Our data suggest that all of the cases can be broadly classified into one of three groups: Wuhan exposure, local transmission and overseas imports. We classify all genomes into 7 clusters. Comparisons reveal higher genomic diversity in the imported group compared to the Wuhan exposure and local transmission, indicating continuous evolution during global transmission.

We quantify SARS-CoV-2 viral load from participants with a diverse range of COVID-19 disease severity, including those requiring hospitalization, outpatients with mild disease, and individuals with resolved infection. We detected SARS-CoV-2 plasma RNA in 27% of hospitalized participants, and 13% of outpatients diagnosed with COVID-19. Amongst the participants hospitalized with COVID-19, we report that a higher prevalence of detectable SARS-CoV-2 plasma viral load is associated with worse respiratory disease severity, lower absolute lymphocyte counts, and increased markers of inflammation.

Evidence-based public health approaches that minimize the introduction and spread of new SARS-CoV-2 transmission clusters are urgently needed in the United States and other countries struggling with expanding epidemics. Here we analyze 247 full-genome sequences from two nearby communities in Wisconsin, and find surprisingly distinct patterns of viral spread.

76 subjects followed to ~100 days demonstrated marked heterogeneity in antibody duration. Virus-specific IgG decayed substantially in most individuals, but a subset had stable or increasing antibody levels despite similar initial antibody magnitudes. Individuals with increasing responses recovered rapidly from COVID-19 disease, had increased somatic mutations in virus-specific memory B cell antibody genes, and had persistent higher frequencies of previously activated CD4+ T cells.

"An outbreak of COVID-19 in a nursing home in the Netherlands, following an on-site church service held on March 8 th, 2020, triggered an investigation to unravel sources and chain(s) of transmission. ? detailed analysis showed a more complex picture, most consistent with widespread regional circulation of the virus in the weeks before the outbreak, and multiple introductions into the nursing home before the visitor ban. The findings underscore the importance of careful outbreak investigations to understand SARS-CoV-2 transmission to develop evidence-based mitigation measures."

RC-PCR WGS applicability for outbreak analysis was tested on 6 predefined clusters containing samples of healthcare workers and patients. RC-PCR resulted in WGS data for 146 samples. 3 out of 6 suspected clusters were fully confirmed, while in other clusters four healthcare workers were not associated. A previously unknown chain of transmission was confirmed.

We systematically explored genetic polymorphisms associated with the clinical outcomes in SARS-CoV infection in humans. literature search comprised available English papers published in PubMed/Medline and SCOPUS databases following the PRISMA-P guidelines and PICO/AXIS criteria. 29 polymorphisms in 21 genes were identified as associated with SARS-CoV susceptibility/resistance, disease severity, and clinical outcomes predominantly in Asian populations.

Employing phylogenetic methods on Danish genome sequences of SARS-CoV-2, we exemplify how genetic data can be used to trace the introduction of a virus to a country. Our analysis supports the hypothesis that the virus was brought to Denmark by skiers returning from Ischgl. On the other hand, we identify transmission routes which suggest that Denmark was part of a network of countries among which the virus was being transmitted.

Here we present genomic surveillance data on 102 imported cases, which account for 17.2% of the total cases in Beijing. Our data suggest that all of the cases can be broadly classified into one of three groups: Wuhan exposure, local transmission and overseas imports. We classify all genomes into 7 clusters. Comparisons reveal higher genomic diversity in the imported group compared to the Wuhan exposure and local transmission, indicating continuous evolution during global transmission.

We quantify SARS-CoV-2 viral load from participants with a diverse range of COVID-19 disease severity, including those requiring hospitalization, outpatients with mild disease, and individuals with resolved infection. We detected SARS-CoV-2 plasma RNA in 27% of hospitalized participants, and 13% of outpatients diagnosed with COVID-19. Amongst the participants hospitalized with COVID-19, we report that a higher prevalence of detectable SARS-CoV-2 plasma viral load is associated with worse respiratory disease severity, lower absolute lymphocyte counts, and increased markers of inflammation.

Evidence-based public health approaches that minimize the introduction and spread of new SARS-CoV-2 transmission clusters are urgently needed in the United States and other countries struggling with expanding epidemics. Here we analyze 247 full-genome sequences from two nearby communities in Wisconsin, and find surprisingly distinct patterns of viral spread.

76 subjects followed to ~100 days demonstrated marked heterogeneity in antibody duration. Virus-specific IgG decayed substantially in most individuals, but a subset had stable or increasing antibody levels despite similar initial antibody magnitudes. Individuals with increasing responses recovered rapidly from COVID-19 disease, had increased somatic mutations in virus-specific memory B cell antibody genes, and had persistent higher frequencies of previously activated CD4+ T cells.

"An outbreak of COVID-19 in a nursing home in the Netherlands, following an on-site church service held on March 8 th, 2020, triggered an investigation to unravel sources and chain(s) of transmission. ? detailed analysis showed a more complex picture, most consistent with widespread regional circulation of the virus in the weeks before the outbreak, and multiple introductions into the nursing home before the visitor ban. The findings underscore the importance of careful outbreak investigations to understand SARS-CoV-2 transmission to develop evidence-based mitigation measures."

RC-PCR WGS applicability for outbreak analysis was tested on 6 predefined clusters containing samples of healthcare workers and patients. RC-PCR resulted in WGS data for 146 samples. 3 out of 6 suspected clusters were fully confirmed, while in other clusters four healthcare workers were not associated. A previously unknown chain of transmission was confirmed.

We systematically explored genetic polymorphisms associated with the clinical outcomes in SARS-CoV infection in humans. literature search comprised available English papers published in PubMed/Medline and SCOPUS databases following the PRISMA-P guidelines and PICO/AXIS criteria. 29 polymorphisms in 21 genes were identified as associated with SARS-CoV susceptibility/resistance, disease severity, and clinical outcomes predominantly in Asian populations.

Employing phylogenetic methods on Danish genome sequences of SARS-CoV-2, we exemplify how genetic data can be used to trace the introduction of a virus to a country. Our analysis supports the hypothesis that the virus was brought to Denmark by skiers returning from Ischgl. On the other hand, we identify transmission routes which suggest that Denmark was part of a network of countries among which the virus was being transmitted.

Here we present genomic surveillance data on 102 imported cases, which account for 17.2% of the total cases in Beijing. Our data suggest that all of the cases can be broadly classified into one of three groups: Wuhan exposure, local transmission and overseas imports. We classify all genomes into 7 clusters. Comparisons reveal higher genomic diversity in the imported group compared to the Wuhan exposure and local transmission, indicating continuous evolution during global transmission.

We quantify SARS-CoV-2 viral load from participants with a diverse range of COVID-19 disease severity, including those requiring hospitalization, outpatients with mild disease, and individuals with resolved infection. We detected SARS-CoV-2 plasma RNA in 27% of hospitalized participants, and 13% of outpatients diagnosed with COVID-19. Amongst the participants hospitalized with COVID-19, we report that a higher prevalence of detectable SARS-CoV-2 plasma viral load is associated with worse respiratory disease severity, lower absolute lymphocyte counts, and increased markers of inflammation.

Evidence-based public health approaches that minimize the introduction and spread of new SARS-CoV-2 transmission clusters are urgently needed in the United States and other countries struggling with expanding epidemics. Here we analyze 247 full-genome sequences from two nearby communities in Wisconsin, and find surprisingly distinct patterns of viral spread.

76 subjects followed to ~100 days demonstrated marked heterogeneity in antibody duration. Virus-specific IgG decayed substantially in most individuals, but a subset had stable or increasing antibody levels despite similar initial antibody magnitudes. Individuals with increasing responses recovered rapidly from COVID-19 disease, had increased somatic mutations in virus-specific memory B cell antibody genes, and had persistent higher frequencies of previously activated CD4+ T cells.

"An outbreak of COVID-19 in a nursing home in the Netherlands, following an on-site church service held on March 8 th, 2020, triggered an investigation to unravel sources and chain(s) of transmission. ? detailed analysis showed a more complex picture, most consistent with widespread regional circulation of the virus in the weeks before the outbreak, and multiple introductions into the nursing home before the visitor ban. The findings underscore the importance of careful outbreak investigations to understand SARS-CoV-2 transmission to develop evidence-based mitigation measures."