Last data update: Sep 30, 2024. (Total: 47785 publications since 2009)
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Query Trace: Spencer JE [original query] |
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A high-throughput and rapid method for accurate identification of emerging multidrug-resistant Candida auris.
Ahmad A , Spencer JE , Lockhart SR , Singleton S , Petway DJ , Bagarozzi DAJr , Herzegh OT . Mycoses 2019 62 (6) 513-518 Candida auris is an emerging multidrug-resistant yeast associated with invasive infection in healthcare settings. Recently, C. auris cases in the US have been detected in 11 states with the majority of cases in New York, New Jersey, and Illinois. Rapid and accurate identification of C. auris is critical for patient care and the implementation of public health measures to control the spread of infection. Our aim was to develop and validate a rapid DNA extraction method using the Roche MagNA Pure 96 instrument and a TaqMan real-time PCR assay for reliable, high throughput identification of C. auris. We evaluated 247 patient dermal swab samples previously analyzed by culture/MALDI-TOF. The diagnostic sensitivity and specificity were 93.6% and 97.2%, respectively. The assay was highly reproducible with a detection limit of 1 C. auris CFU/10muL. A ROC curve analysis of the real-time PCR data showed an area of 0.982 under the curve, with a CT cutoff value of </=37.0. The turnaround time from DNA extraction to real-time PCR results was approximately 200 samples/day. In conclusion, we successfully validated a rapid and high throughput method for accurate and reproducible identification of C. auris with a significantly reduced turnaround time compared to culture/MALDI-TOF based methods. This article is protected by copyright. All rights reserved. |
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