Last data update: Jul 01, 2024. (Total: 47134 publications since 2009)
Records 1-5 (of 5 Records) |
Query Trace: Gonzalez GL [original query] |
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Genomic surveillance of SARS-CoV-2 in Puerto Rico reveals emergence of an autochthonous lineage and early detection of variants (preprint)
Santiago GA , Flores B , Gonzalez GL , Charriez KN , Cora-Huertas L , Volkman HR , Van Belleghem S , Rivera-Amill V , Adams LE , Marzan M , Hernandez L , Cardona I , O'Neill E , Paz-Bailey G , Papa R , Munoz-Jordan JL . Res Sq 2022 Puerto Rico has experienced the full impact of the COVID-19 pandemic. Since SARS-CoV-2, the virus that causes COVID-19, was first detected on the island in March of 2020, it spread rapidly though the island’s population and became a critical threat to public health. We conducted a genomic surveillance study through a partnership with health agencies and academic institutions to understand the emergence and molecular epidemiology of the virus on the island. We sampled COVID-19 cases monthly over 19 months and sequenced a total of 753 SARS-CoV-2 genomes between March 2020 and September 2021 to reconstruct the local epidemic in a regional context using phylogenetic inference. Our analyses revealed that multiple importation events propelled the emergence and spread of the virus throughout the study period, including the introduction and spread of most SARS-CoV-2 variants detected world-wide. Lineage turnover cycles through various phases of the local epidemic were observed, where the predominant lineage was replaced by the next competing lineage or variant after approximately 4 months of circulation locally. We also identified the emergence of lineage B.1.588, an autochthonous lineage that predominated circulation in Puerto Rico from September to December 2020 and subsequently spread to the United States. The results of this collaborative approach highlight the importance of timely collection and analysis of SARS-CoV-2 genomic surveillance data to inform public health responses. |
SARS-CoV-2 Omicron replacement of Delta as predominant variant, Puerto Rico
Santiago GA , Volkman HR , Flores B , González GL , Charriez KN , Huertas LC , Van Belleghem SM , Rivera-Amill V , Major C , Colon C , Tosado R , Adams LE , Marzán M , Hernández L , Cardona I , O'Neill E , Paz-Bailey G , Papa R , Muñoz-Jordan JL . Emerg Infect Dis 2023 29 (4) 855-857 ![]() We reconstructed the SARS-CoV-2 epidemic caused by Omicron variant in Puerto Rico by sampling genomes collected during October 2021-May 2022. Our study revealed that Omicron BA.1 emerged and replaced Delta as the predominant variant in December 2021. Increased transmission rates and a dynamic landscape of Omicron sublineage infections followed. |
Genomic surveillance of SARS-CoV-2 in Puerto Rico enabled early detection and tracking of variants.
Santiago GA , Flores B , González GL , Charriez KN , Huertas LC , Volkman HR , Van Belleghem SM , Rivera-Amill V , Adams LE , Marzán M , Hernández L , Cardona I , O'Neill E , Paz-Bailey G , Papa R , Muñoz-Jordan JL . Commun Med (Lond) 2022 2 100 ![]() ![]() BACKGROUND: Puerto Rico has experienced the full impact of the COVID-19 pandemic. Since SARS-CoV-2, the virus that causes COVID-19, was first detected on the island in March of 2020, it spread rapidly though the island's population and became a critical threat to public health. METHODS: We conducted a genomic surveillance study through a partnership with health agencies and academic institutions to understand the emergence and molecular epidemiology of the virus on the island. We sampled COVID-19 cases monthly over 19 months and sequenced a total of 753 SARS-CoV-2 genomes between March 2020 and September 2021 to reconstruct the local epidemic in a regional context using phylogenetic inference. RESULTS: Our analyses reveal that multiple importation events propelled the emergence and spread of the virus throughout the study period, including the introduction and spread of most SARS-CoV-2 variants detected world-wide. Lineage turnover cycles through various phases of the local epidemic were observed, where the predominant lineage was replaced by the next competing lineage or variant after ~4 months of circulation locally. We also identified the emergence of lineage B.1.588, an autochthonous lineage that predominated in Puerto Rico from September to December 2020 and subsequently spread to the United States. CONCLUSIONS: The results of this collaborative approach highlight the importance of timely collection and analysis of SARS-CoV-2 genomic surveillance data to inform public health responses. |
Tracing the Origin, Spread, and Molecular Evolution of Zika Virus in Puerto Rico, 2016-2017.
Santiago GA , Kalinich CC , Cruz-López F , González GL , Flores B , Hentoff A , Charriez KN , Fauver JR , Adams LE , Sharp TM , Black A , Bedford T , Ellis E , Ellis B , Waterman SH , Paz-Bailey G , Grubaugh ND , Muñoz-Jordán JL . Emerg Infect Dis 2021 27 (11) 2971-2973 ![]() ![]() We reconstructed the 2016-2017 Zika virus epidemic in Puerto Rico by using complete genomes to uncover the epidemic's origin, spread, and evolutionary dynamics. Our study revealed that the epidemic was propelled by multiple introductions that spread across the island, intricate evolutionary patterns, and ≈10 months of cryptic transmission. |
Development of a standardized Sanger-based method for partial sequencing and genotyping dengue viruses.
Santiago GA , Gonzalez GL , Cruz-Lopez F , Munoz-Jordan JL . J Clin Microbiol 2019 57 (4) ![]() The global expansion of dengue viruses (DENV-1-4) has contributed to the divergence, transmission, and establishment of genetic lineages of epidemiological concern; however, tracking phylogenetic relationships of these virus is not always possible due to inability of standardized sequencing procedures in resource-limited public health laboratories Consequently, public genomic data banks contain inadequate representation of geographical regions and historical periods. In order to improve detection of DENV-1-4 lineages, we report the development of a serotype-specific Sanger-based method standardized to sequence DENV-1-4 directly from clinical samples using universal primers that detect most DENV genotypes. The resulting envelope protein coding sequences are analyzed for genotyping with phylogenetic methods. We evaluated the performance of this method by detecting, amplifying, and sequencing 54 contemporary DENV isolates, including 29 clinical samples, representing a variety of genotypes of epidemiological importance and global presence. All specimens were sequenced successfully and phylogenetic reconstructions resulted in the expected genotype classification. To further improve genomic surveillance in dengue endemic regions, this method was transferred to 16 public health laboratories in 13 Latin American countries, to date. Our objective is to provide an accessible method that facilitates the integration of genomics with dengue surveillance. |
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