Clinical Performance and Trends During the First Two Months of Monkeypox Virus PCR Testing at Two United States Reference Labs
NAP Lieberman et al, MEDRXIV, September 21, 2022
We examine epidemiological characteristics, specimen collection practices, and cycle threshold (Ct) values for MPXV PCR tests performed at two reference laboratories. Results from both laboratories support public health data showing a high positivity rate in men (>30%) and those ages 30-49 (25-35%). The overall positivity rate decreased during the study period but remains elevated (~20%). There was a significant difference in Ct values between laboratories (ARUP 23.86 vs. UW 25.40) and collection method (22.79 for dry swab vs. 24.44 for VTM).
Wastewater based epidemiology beyond SARS-CoV-2: Spanish wastewater reveals the current spread of Monkeypox virus
G Guzman et al, MEDRXIV, September 19, 2022
This study shows that MPXV DNA can be reproducibly detected by qPCR in longitudinal samples collected from different Spanish wastewater treatment plants. According to data from the National Epidemiological Surveillance Network (RENAVE) in Spain a total of 6,119 cases have been confirmed as of August 19, 2022. However, and based on the wastewater data, the reported clinical cases seem to be underestimated and asymptomatic infections may be more frequent than expected.
Wastewater Surveillance for Monkeypox Virus in Nine California Communities
MK Wolfe et al, MEDRXIV, September 9, 2022
MPXV DNA was detected at all nine sites between June 19 and August 1, 2022; 5 of 9 sites detected MPXV DNA prior to or within a day of the first case identified in the source sewershed. At the four sites with >10 positive detections, we observed a positive, statistically significant correlation (p <0.001) between MPXV DNA in wastewater solids and incidence rate of reported cases.
First detection of the Monkeypox virus using wastewater-based surveillance in Miami-Dade County
ME Sharkey al, Research Square, September 1, 2022
A similar situation early in the COVID-19 pandemic led to expansion of efforts with sampling of wastewater as an alternative means of clinical testing for community surveillance. Here we discuss an opportunity to use similar sampling processes for detection of MPXV. Recent data from different body fluids suggests shedding may occur via multiple body sites as the virus has been detected by PCR in saliva, semen, urine, and feces, fluids which may ultimately be potentially discarded in the wastewater.