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Last Posted: Oct 26, 2023
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Use of Wastewater for Mpox Outbreak Surveillance in California.
Wolfe Marlene K et al. The New England journal of medicine 2023 1

As part of the response to the spread of MPXV infection, we adapted and deployed polymerase-chain-reaction assays targeting MPXV genomic DNA5 as part of our ongoing wastewater surveillance program. Testing was implemented within a month after the first identified case of MPXV infection in the United States, and the results were used in real time for the public health response in California.

Development of an amplicon-based sequencing approach in response to the global emergence of human monkeypox virus
NFG Chen et al, MEDRXIV, January 13, 2023

Highly multiplexed amplicon-based sequencing (PrimalSeq) was initially developed for sequencing of Zika virus, and later adapted as the main sequencing approach for SARS-CoV-2. Here, we used PrimalScheme to develop a primer scheme for human monkeypox virus that can be used with many sequencing and bioinformatics pipelines implemented in public health laboratories during the COVID-19 pandemic. We sequenced clinical samples that tested presumptive positive for human monkeypox virus with amplicon-based and metagenomic sequencing approaches.

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