Last data update: Sep 16, 2024. (Total: 47680 publications since 2009)
Records 1-5 (of 5 Records) |
Query Trace: Volkov B [original query] |
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Evaluating application of knowledge and skills: the use of consensus expert review to assess conference abstracts of Field Epidemiology Training participants
Volkov B , MacDonald G , Herrera-Guibert D , Jones D , Patel M . J Multidiscip Eval 2014 10 (23) 44-50 Background: Often evaluations of training programs are limited - with many focusing on the aspects that are easy to measure (e.g., reaction of trainees) without addressing the important outcomes of training, such as how trainees applied their new knowledge, skills, and attitudes. Numerous evaluations fail to measure training’s effect on job performance because few effective methods are available to do so. Particularly difficult is the problem of evaluating multisite training programs that vary considerably in structure and implementation from one site to another. Research Design: We devised a method of a consensus expert review to evaluate the quality of conference abstracts submitted by participants in Field Epidemiology Training Programs - an approach that can provide useful information on how well trainees apply knowledge and skills gained in training, complementing data obtained from other sources and methods. This method is practical, minimally intrusive, and resource-efficient, and it may prove useful for evaluation practice in diverse fields that require training. |
Reducing health care costs for dementia patients: estimating savings from a caregiver support program
Klug MG , Muus K , Volkov B , Halaas GW . J Aging Health 2012 24 (8) 1470-85 OBJECTIVE: Estimating cost savings based on limited health care events within a short time span for a dementia care program. METHOD: Data on health care utilization of persons with dementia (PWDs) and caregivers were gathered in the Dementia Care Services Program in North Dakota from January 2010 to January 2012. Data were aggregated into 3-month intervals and compared to 3 months before program intervention. Paired and cross-time models were used to estimate cost savings. RESULTS: Health care cost savings for PWDs were estimated at US$143,118 to US$180,102 during the first 3 months after intervention, then decreased over time. Only the first 9 months could be used in the paired model due to small N and low power. DISCUSSION: For programs with short time spans and limited health care events, a cross-time model can be used to estimate cost savings while producing results similar to paired models. |
Citrullination of proteins: a common post-translational modification pathway induced by different nanoparticles in vitro and in vivo
Mohamed BM , Verma NK , Davies AM , McGowan A , Staunton KC , Prina-Mello A , Kelleher D , Botting CH , Causey CP , Thompson PR , Pruijn GJ , Kisin ER , Tkach AV , Shvedova AA , Volkov Y . Nanomedicine (London) 2012 7 (8) 1181-95 AIM: Rapidly expanding manufacture and use of nanomaterials emphasize the requirements for thorough assessment of health outcomes associated with novel applications. Post-translational protein modifications catalyzed by Ca(2+)-dependent peptidylargininedeiminases have been shown to trigger immune responses including autoantibody generation, a hallmark of immune complexes deposition in rheumatoid arthritis. Therefore, the aim of the study was to assess if nanoparticles are able to promote protein citrullination. MATERIALS & METHODS: Human A549 and THP-1 cells were exposed to silicon dioxide, carbon black or single-walled carbon nanotubes. C57BL/6 mice were exposed to respirable single-walled carbon nanotubes. Protein citrullination, peptidylargininedeiminases activity and target proteins were evaluated. RESULTS: The studied nanoparticles induced protein citrullination both in cultured human cells and mouse lung tissues. Citrullination occurred via the peptidylargininedeiminase-dependent mechanism. Cytokeratines 7, 8, 18 and plectins were identified as intracellular citrullination targets. CONCLUSION: Nanoparticle exposure facilitated post-translational citrullination of proteins. Original submitted 18 March 2011; Revised submitted 10 Novemeber 2011. |
Impaired clearance and enhanced pulmonary inflammatory/fibrotic response to carbon nanotubes in myeloperoxidase-deficient mice
Shvedova AA , Kapralov AA , Feng WH , Kisin ER , Murray AR , Mercer RR , St Croix CM , Lang MA , Watkins SC , Konduru NV , Allen BL , Conroy J , Kotchey GP , Mohamed BM , Meade AD , Volkov Y , Star A , Fadeel B , Kagan VE . PLoS One 2012 7 (3) e30923 Advancement of biomedical applications of carbonaceous nanomaterials is hampered by their biopersistence and pro-inflammatory action in vivo. Here, we used myeloperoxidase knockout B6.129X1-MPO (MPO k/o) mice and showed that oxidation and clearance of single walled carbon nanotubes (SWCNT) from the lungs of these animals after pharyngeal aspiration was markedly less effective whereas the inflammatory response was more robust than in wild-type C57Bl/6 mice. Our results provide direct evidence for the participation of MPO - one of the key-orchestrators of inflammatory response - in the in vivo pulmonary oxidative biodegradation of SWCNT and suggest new ways to control the biopersistence of nanomaterials through genetic or pharmacological manipulations. |
Carbon nanotubes degraded by neutrophil myeloperoxidase induce less pulmonary inflammation
Kagan VE , Konduru NV , Feng W , Allen BL , Conroy J , Volkov Y , Vlasova II , Belikova NA , Yanamala N , Kapralov A , Tyurina YY , Shi J , Kisin ER , Murray AR , Franks J , Stolz D , Gou P , Klein-Seetharaman J , Fadeel B , Star A , Shvedova AA . Nat Nanotechnol 2010 5 (5) 354-9 We have shown previously that single-walled carbon nanotubes can be catalytically biodegraded over several weeks by the plant-derived enzyme, horseradish peroxidase. However, whether peroxidase intermediates generated inside human cells or biofluids are involved in the biodegradation of carbon nanotubes has not been explored. Here, we show that hypochlorite and reactive radical intermediates of the human neutrophil enzyme myeloperoxidase catalyse the biodegradation of single-walled carbon nanotubes in vitro, in neutrophils and to a lesser degree in macrophages. Molecular modelling suggests that interactions of basic amino acids of the enzyme with the carboxyls on the carbon nanotubes position the nanotubes near the catalytic site. Importantly, the biodegraded nanotubes do not generate an inflammatory response when aspirated into the lungs of mice. Our findings suggest that the extent to which carbon nanotubes are biodegraded may be a major determinant of the scale and severity of the associated inflammatory responses in exposed individuals. |
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