Last data update: Jun 11, 2024. (Total: 46992 publications since 2009)
Records 1-3 (of 3 Records) |
Query Trace: Shogren ES [original query] |
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Pulmonary inflammation induced by office dust and the relation to 3-beta-glucan using different extraction techniques
Young S , Cox-Ganser JM , Shogren ES , Wolfarth MG , Li S , Antonini JM , Castranova V , Park J . Toxicol Environ Chem 2011 93 (4) 806-823 It is observed that 3-beta-glucan, a major cell wall component of fungi, induces pulmonary inflammation. There is inconsistency in determining the correlation between the levels of glucan measured by current extraction methods and the respiratory inflammation observed in individuals or lab animals exposed to environmental dust samples. The glucan-specific limulus amebocyte lysate (G-LAL) method was used after extraction with dimethyl sulfoxide (DMSO) or sodium hydroxide (NaOH) to analyze the glucan content of office dust samples collected from a water-damaged building. C3HeB/FeJ mice, an endotoxinsensitive strain, were treated with different dust samples (2.5 mg kg-1 body weight) or saline (vehicle control) by pharyngeal aspiration. At 1 day after aspiration, bronchoalveolar lavage (BAL) was performed, and lung inflammation and injury were assessed by measuring: (1) neutrophil (PMN) infiltration, (2) inflammatory cytokine (IL-6, IL-10, MCP-1, IFN-, TNF-, and IL12-p70) levels, and (3) albumin and lactate dehydrogenase in recovered BAL fluid. Both DMSO and NaOH extraction increased the detection of glucan by approximately 20-fold compared to water extraction. However, only the DMSO extraction method showed a statistically significant positive correlation between 13-beta-glucan and albumin levels, total numbers of BAL, polymorphonuclear leukocytes (PMNs) cells recovered, levels of TNF-, MCP-1, and IL-6. In conclusion, 3-beta-glucan is a potent inflammatory agent in dust samples and DMSO extraction for glucan analysis may prove useful in understanding the impact of environmental contamination by glucans on lung disease. 2011 Taylor Francis. |
Pre-sampling contamination of filters used in measurements of airborne (1 → 3)-β-D-glucan based on glucan-specific Limulus amebocyte lysate assay
Shogren ES , Park JH . J Environ Monit 2011 13 (4) 1082-7 Air sampling for (1 --> 3)-beta-D-glucan may be a good method for assessing inhalation exposure to airborne fungi. Pre-sampling contamination of filter media used for sampling (1 --> 3)-beta-D-glucan may lead to substantial exposure measurement errors. Using the Limulus amebocyte lysate assay, we tested for pre-sampling levels of (1 --> 3)-beta-D-glucan on three types of filters-mixed cellulose ester (MCE)[1 brand], glass fiber (GF)[1 brand], and polycarbonate (PC)[5 brands]. Levels of (1 --> 3)-beta-D-glucan on MCE filters exceeded 4586.1 pg per filter. Levels on GF filters averaged 135.3 (+/-28.9) pg per filter (range = 94.8-160.4 pg per filter) and levels on PC filters averaged 152.4 (+/-236.1) pg per filter (range = non-detectable-1760.7 pg per filter). Efforts to clean MCE and GF filters were unfeasible or unsuccessful. Sonicating PC filters for two hours in ethanol, followed by a wash in pyrogen-free water, effectively eliminated measured levels of (1 --> 3)-beta-D-glucan on four brands of PC filters, as compared to untreated PC filters. This pretreatment process did not appear to physically damage the PC filters. Air sampling results highlighted the potentially problematic contamination of untreated PC filters. Ensuring that sampling media are free of (1 --> 3)-beta-D-glucan before sampling is crucial to accurately measure levels of (1 --> 3)-beta-D-glucan exposure, especially in environments where levels of (1 --> 3)-beta-D-glucan are low. |
Prospective comparison of tuberculin skin test and QuantiFERON-TB Gold In-Tube assay for the detection of latent tuberculosis infection among healthcare workers in a low-incidence setting
Cummings KJ , Smith TS , Shogren ES , Khakoo R , Nanda S , Bunner L , Smithmyer A , Soccorsi D , Kashon ML , Mazurek GH , Friedman LN , Weissman DN . Infect Control Hosp Epidemiol 2009 30 (11) 1123-6 We compared the results of the tuberculin skin test with the results of the QuantiFERON-TB Gold In-Tube (QFT-GIT) assay among 182 low-risk healthcare workers. Overall agreement and specificity were high, but the tests did not agree on positive results. Only 2 of 5 positive QFT-GIT assay results could be confirmed with repeat analyses. Indeterminate results were associated with potential immunosuppression. |
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