Last data update: Jan 27, 2025. (Total: 48650 publications since 2009)
Records 1-8 (of 8 Records) |
Query Trace: Santovenia M[original query] |
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Comparative genomics of Salmonella enterica serovar Montevideo reveals lineage-specific gene differences that may influence ecological niche association.
Nguyen SV , Harhay DM , Bono JL , Smith TPL , Fields PI , Dinsmore BA , Santovenia M , Wang R , Bosilevac JM , Harhay GP . Microb Genom 2018 4 (8) ![]() ![]() Salmonella enterica serovar Montevideo has been linked to recent foodborne illness outbreaks resulting from contamination of products such as fruits, vegetables, seeds and spices. Studies have shown that Montevideo also is frequently associated with healthy cattle and can be isolated from ground beef, yet human salmonellosis outbreaks of Montevideo associated with ground beef contamination are rare. This disparity fuelled our interest in characterizing the genomic differences between Montevideo strains isolated from healthy cattle and beef products, and those isolated from human patients and outbreak sources. To that end, we sequenced 13 Montevideo strains to completion, producing high-quality genome assemblies of isolates from human patients (n=8) or from healthy cattle at slaughter (n=5). Comparative analysis of sequence data from this study and publicly available sequences (n=72) shows that Montevideo falls into four previously established clades, differentially occupied by cattle and human strains. The results of these analyses reveal differences in metabolic islands, environmental adhesion determinants and virulence factors within each clade, and suggest explanations for the infrequent association between bovine isolates and human illnesses. |
Notes from the Field: Vibrio cholerae Serogroup O1, Serotype Inaba - Minnesota, August 2016
Hall V , Medus C , Wahl G , Sorenson A , Orth M , Santovenia M , Burdette E , Smith K . MMWR Morb Mortal Wkly Rep 2017 66 (36) 961-962 On August 20, 2016, the Minnesota Department of Health (MDH) was notified of a case of Vibrio cholerae infection. The isolate was identified as serogroup O1, serotype Inaba at MDH. CDC determined that the isolate was nontoxigenic. The patient was a previously healthy woman, aged 43 years, with history of gastric bypass surgery. On August 16, she experienced profuse watery diarrhea, vomiting, abdominal cramps, and headache. On August 18, she sought care and submitted the stool specimen that yielded the V. cholerae isolate. She reported no recent travel. However, she had consumed ceviche made with raw shrimp and raw oysters at restaurant A on August 14, 49 hours before illness onset. Her husband had a similar illness with a similar incubation period after eating the same foods at restaurant A. | On August 22, MDH sanitarians visited restaurant A and obtained tags and invoices for oyster and shrimp products; the oysters were a product of the United States, and the shrimp was a product of India. Sanitarians also gathered patron contact information and credit card receipts for August 12–14. Two additional patrons reported experiencing a gastrointestinal illness that met the case definition of three or more episodes of watery stool in a 24-hour period within 5 days of eating at restaurant A; one reported eating ceviche and oysters at restaurant A. Review of complaints to the MDH foodborne illness hotline revealed a previous complaint from two persons who reported experiencing watery diarrhea after eating raw shrimp ceviche (but no oysters) at restaurant A on August 2. These persons did not provide stool specimens, but their gastrointestinal illnesses met the case definition, resulting in a total of six cases, including one laboratory-confirmed case. No other V. cholerae O1 Inaba cases were reported in the United States during this outbreak. |
Complete, Closed Genome Sequences of 10 Salmonella enterica subsp. enterica Serovar Typhimurium Strains Isolated from Human and Bovine Sources.
Nguyen SV , Harhay DM , Bono JL , Smith TP , Fields PI , Dinsmore BA , Santovenia M , Kelley CM , Wang R , Bosilevac JM , Harhay GP . Genome Announc 2016 4 (6) ![]() Salmonella enterica is a leading cause of enterocolitis for humans and animals. S. enterica subsp. enterica serovar Typhimurium infects a broad range of hosts. To facilitate genomic comparisons among isolates from different sources, we present the complete genome sequences of 10 S Typhimurium strains, 5 each isolated from human and bovine sources. |
Complete and Closed Genome Sequences of 10 Salmonella enterica subsp. enterica Serovar Anatum Isolates from Human and Bovine Sources.
Nguyen SV , Harhay DM , Bono JL , Smith TP , Fields PI , Dinsmore BA , Santovenia M , Kelley CM , Wang R , Bosilevac JM , Harhay GP . Genome Announc 2016 4 (3) ![]() Salmonella enterica is an important pathogen transmitted by numerous vectors. Genomic comparisons of Salmonella strains from disparate hosts have the potential to further our understanding of mechanisms underlying host specificities and virulence. Here, we present the closed genome and plasmid sequences of 10 Salmonella enterica subsp. enterica serovar Anatum isolates from bovine and human sources. |
Multicenter evaluation of clinical diagnostic methods for detection and isolation of Campylobacter spp. from stool
Fitzgerald C , Patrick M , Gonzalez A , Akin J , Polage CR , Wymore K , Gillim-Ross L , Xavier K , Sadlowski J , Monahan J , Hurd S , Dahlberg S , Jerris R , Watson R , Santovenia M , Mitchell D , Harrison C , Tobin-D'Angelo M , DeMartino M , Pentella M , Razeq J , Leonard C , Jung C , Achong-Bowe R , Evans Y , Jain D , Juni B , Leano F , Robinson T , Smith K , Gittelman RM , Garrigan C , Nachamkin I . J Clin Microbiol 2016 54 (5) 1209-15 ![]() The use of culture independent diagnostic tests (CIDTs), such as stool antigen tests, as standalone tests for the detection of Campylobacter in stool is increasing. We conducted a prospective, multicenter study to evaluate the performance of stool antigen CIDTs compared to culture and PCR for Campylobacter detection. Between July and October, 2010, we tested 2,767 stool specimens from patients with gastrointestinal illness with the following methods: four types of Campylobacter selective media, four commercial stool antigen assays, and a commercial PCR assay. Illnesses from which specimens were positive by one or more culture media or at least one CIDT and PCR were designated as 'cases'. A total of 95 specimens (3.4 %) met the case definition. The sensitivity/specificity/positive predictive values of the stool antigen CIDTs ranged from 79.6%-87.6% in sensitivity, 95.9 - 99.5% specificity, and 41.3 - 84.3% positive predictive value. Culture alone detected 80/89 (89.9% sensitivity) C. jejuni/C. coli positive cases. Of the 209 non-cases that were positive by at least one CIDT, only one (0.48%) was positive by all four stool antigen tests, and 73% were positive by just one stool antigen test. The questionable relevance of unconfirmed positive stool antigen CIDT results was supported by the finding that non-cases were less likely than cases to have gastrointestinal symptoms. Thus, while convenient to use, the sensitivity, specificity and positive predictive value of Campylobacter stool antigen tests were highly variable. Given the relatively low incidence of Campylobacter disease and the generally poor diagnostic test characteristics, this study calls into question the use of commercially available stool antigen CIDT tests as standalone tests for direct detection of Campylobacter in stool. |
Complete Closed Genome Sequences of Salmonella enterica subsp. enterica Serotypes Anatum, Montevideo, Typhimurium, and Newport, Isolated from Beef, Cattle, and Humans.
Harhay DM , Bono JL , Smith TP , Fields PI , Dinsmore BA , Santovenia M , Kelley CM , Wang R , Harhay GP . Genome Announc 2016 4 (1) ![]() Salmonella enterica spp. are a diverse group of bacteria with a wide range of virulence potential. To facilitate genome comparisons across this virulence spectrum, we present eight complete closed genome sequences of four S. enterica serotypes (Anatum, Montevideo, Typhimurium, and Newport), isolated from various cattle samples and from humans. |
Campylobacter fetus subsp. testudinum subsp. nov., isolated from humans and reptiles.
Fitzgerald C , Tu ZC , Patrick M , Stiles T , Lawson AJ , Santovenia M , Gilbert MJ , van Bergen M , Joyce K , Pruckler J , Stroika S , Duim B , Miller WG , Loparev VL , Sinnige JC , Fields PI , Tauxe RV , Blaser MJ , Wagenaar JA . Int J Syst Evol Microbiol 2014 64 2944-2948 ![]() A polyphasic study was undertaken to determine the taxonomic position of 13 Campylobacter fetus-like strains from humans (n=8) and reptiles (n=5). The results of matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) and genomic data from sap analysis, 16S rRNA and hsp60 sequence comparison, pulsed- field gel electrophoresis (PFGE), amplified fragment length polymorphism (AFLP) analysis, DNA-DNA hybridization and whole genome sequencing demonstrate that these strains are closely related to C. fetus but clearly differentiated from the present C. fetus subspecies. Therefore this unique cluster of 13 strains represents a novel subspecies within the species C. fetus, for which the name Campylobacter fetus subsp. testudinum subsp. nov. is proposed, with strain 03-427T (=ATCC BAA-2539T = LMG 27499T) as the type strain. Although this novel taxon cannot be differentiated from C. fetus subsp. fetus and C. fetus subsp. venerealis using conventional phenotypic tests, MALDI-TOF MS analysis revealed the presence of multiple phenotypic biomarkers which distinguish C. fetus subsp. testudinum from the present C. fetus subspecies. |
Multilocus sequence typing confirms wild birds as the source of a Campylobacter outbreak associated with the consumption of raw peas.
Kwan PS , Xavier C , Santovenia M , Pruckler J , Stroika S , Joyce K , Gardner T , Fields PI , McLaughlin J , Tauxe RV , Fitzgerald C . Appl Environ Microbiol 2014 80 (15) 4540-6 ![]() From August to September 2008, the Centers for Disease Control and Prevention (CDC) assisted the Alaska Division of Public Health with an outbreak investigation of campylobacteriosis occurring among the residents of Southcentral Alaska. During the investigation, pulsed-field gel electrophoresis (PFGE) of Campylobacter jejuni isolates from human, raw pea and wild bird fecal samples confirmed the epidemiologic link between illness and the consumption of raw peas contaminated by Sandhill cranes from 15 of 43 epidemiologically-linked human isolates. However, an association between the remaining epidemiologically-linked human infections and the pea and wild bird isolates was not established. To better understand the molecular epidemiology of the outbreak, C. jejuni isolates (n=130; 59 humans, 40 peas and 31 wild birds) were further characterized by multi-locus sequence typing (MLST). Here, we present the molecular evidence to demonstrate the association of many more human C. jejuni infections associated with the outbreak with raw peas and wild bird feces. Among all sequence types (STs) identified, 26 (67%) of 39 were novel and exclusive to the outbreak. Five clusters of overlapping STs (n=32 isolates; 17 humans, 2 peas and 13 wild birds) were identified. In particular, Cluster E (n=7 isolates, ST-5049) consisted of isolates from humans, peas and wild birds. Novel STs clustered closely with isolates typically associated with wild birds and the environment, but distinct from lineages commonly seen in human infections. Novel STs and alleles recovered from human outbreak isolates allowed additional infections caused by these rare genotypes to be attributed to the contaminated raw peas. |
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