Last data update: Jun 17, 2024. (Total: 47034 publications since 2009)
Records 1-3 (of 3 Records) |
Query Trace: Reed ZD [original query] |
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Seasonal pulses of Marburg virus circulation in juvenile Rousettus aegyptiacus bats coincide with periods of increased risk of human infection
Amman BR , Carroll SA , Reed ZD , Sealy TK , Balinandi S , Swanepoel R , Kemp A , Erickson BR , Comer JA , Campbell S , Cannon DL , Khristova ML , Atimnedi P , Paddock CD , Kent Crockett RJ , Flietstra TD , Warfield KL , Unfer R , Katongole-Mbidde E , Downing R , Tappero JW , Zaki SR , Rollin PE , Ksiazek TG , Nichol ST , Towner JS . PLoS Pathog 2012 8 (10) e1002877 ![]() Marburg virus (family Filoviridae) causes sporadic outbreaks of severe hemorrhagic disease in sub-Saharan Africa. Bats have been implicated as likely natural reservoir hosts based most recently on an investigation of cases among miners infected in 2007 at the Kitaka mine, Uganda, which contained a large population of Marburg virus-infected Rousettus aegyptiacus fruit bats. Described here is an ecologic investigation of Python Cave, Uganda, where an American and a Dutch tourist acquired Marburg virus infection in December 2007 and July 2008. More than 40,000 R. aegyptiacus were found in the cave and were the sole bat species present. Between August 2008 and November 2009, 1,622 bats were captured and tested for Marburg virus. Q-RT-PCR analysis of bat liver/spleen tissues indicated approximately 2.5% of the bats were actively infected, seven of which yielded Marburg virus isolates. Moreover, Q-RT-PCR-positive lung, kidney, colon and reproductive tissues were found, consistent with potential for oral, urine, fecal or sexual transmission. The combined data for R. aegyptiacus tested from Python Cave and Kitaka mine indicate low level horizontal transmission throughout the year. However, Q-RT-PCR data show distinct pulses of virus infection in older juvenile bats ( approximately six months of age) that temporarily coincide with the peak twice-yearly birthing seasons. Retrospective analysis of historical human infections suspected to have been the result of discrete spillover events directly from nature found 83% (54/65) events occurred during these seasonal pulses in virus circulation, perhaps demonstrating periods of increased risk of human infection. The discovery of two tags at Python Cave from bats marked at Kitaka mine, together with the close genetic linkages evident between viruses detected in geographically distant locations, are consistent with R. aegyptiacus bats existing as a large meta-population with associated virus circulation over broad geographic ranges. These findings provide a basis for developing Marburg hemorrhagic fever risk reduction strategies. |
Serology and cytokine profiles in patients infected with the newly discovered Bundibugyo ebolavirus
Gupta M , Macneil A , Reed ZD , Rollin PE , Spiropoulou CF . Virology 2012 423 (2) 119-24 A new species of Ebolavirus, Bundibugyo ebolavirus, was discovered in an outbreak in western Uganda in November 2007. To study the correlation between fatal infection and immune response in Bundibugyo ebolavirus infection, viral antigen, antibodies, and 17 soluble factors important for innate immunity were examined in 44 patient samples. Using Luminex assays, we found that fatal infection was associated with high levels of viral antigen, low levels of pro-inflammatory cytokines, such as IL-1alpha, IL-1beta, IL-6, TNF-alpha, and high levels of immunosuppressor cytokines like IL-10. Also, acute infected patients died in spite of generating high levels of antibodies against the virus. Thus, our results imply that disease severity in these patients is not due to the multi-organ failure and septic shock caused by a flood of inflammatory cytokines, as seen in infections with other Ebolavirus species. |
Isolation of genetically diverse Marburg viruses from Egyptian fruit bats
Towner JS , Amman BR , Sealy TK , Carroll SA , Comer JA , Kemp A , Swanepoel R , Paddock CD , Balinandi S , Khristova ML , Formenty PB , Albarino CG , Miller DM , Reed ZD , Kayiwa JT , Mills JN , Cannon DL , Greer PW , Byaruhanga E , Farnon EC , Atimnedi P , Okware S , Katongole-Mbidde E , Downing R , Tappero JW , Zaki SR , Ksiazek TG , Nichol ST , Rollin PE . PLoS Pathog 2009 5 (7) e1000536 ![]() In July and September 2007, miners working in Kitaka Cave, Uganda, were diagnosed with Marburg hemorrhagic fever. The likely source of infection in the cave was Egyptian fruit bats (Rousettus aegyptiacus) based on detection of Marburg virus RNA in 31/611 (5.1%) bats, virus-specific antibody in bat sera, and isolation of genetically diverse virus from bat tissues. The virus isolates were collected nine months apart, demonstrating long-term virus circulation. The bat colony was estimated to be over 100,000 animals using mark and re-capture methods, predicting the presence of over 5,000 virus-infected bats. The genetically diverse virus genome sequences from bats and miners closely matched. These data indicate common Egyptian fruit bats can represent a major natural reservoir and source of Marburg virus with potential for spillover into humans. |
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