Last data update: Apr 29, 2024. (Total: 46658 publications since 2009)
Records 1-2 (of 2 Records) |
Query Trace: Randall JM [original query] |
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Comparison of platforms for testing antibodies to Chlamydia trachomatis antigens in the Democratic Republic of the Congo and Togo
Gwyn S , Awoussi MS , Bakhtiari A , Bronzan RN , Crowley K , Harding-Esch EM , Kassankogno Y , Kilangalanga JN , Makangila F , Mupoyi S , Ngondi J , Ngoyi B , Palmer S , Randall JM , Seim A , Solomon AW , Stewart R , Togbey K , Uvon PA , Martin DL . Sci Rep 2021 11 (1) 7225 Trachoma, caused by repeated ocular infection with Chlamydia trachomatis (Ct), is targeted for elimination as a public health problem. Serological testing for antibodies is promising for surveillance; determining useful thresholds will require collection of serological data from settings with different prevalence of the indicator trachomatous inflammation-follicular (TF). Dried blood spots were collected during trachoma mapping in two districts each of Togo and Democratic Republic of the Congo. Anti-Ct antibodies were detected by multiplex bead assay (MBA) and three different lateral flow assays (LFA) and seroprevalence and seroconversion rate (SCR) were determined. By most tests, the district with > 5% TF (the elimination threshold) had five-sixfold higher seroprevalence and tenfold higher SCR than districts with < 5% TF. The agreement between LFA and MBA was improved using a black latex developing reagent. These data show optimization of antibody tests against Ct to better differentiate districts above or below trachoma elimination thresholds. |
Optimization of a rapid test for antibodies to the Chlamydia trachomatis antigen Pgp3
Gwyn S , Mkocha H , Randall JM , Kasubi M , Martin DL . Diagn Microbiol Infect Dis 2018 93 (4) 293-298 Serological surveillance for trachoma could allow monitoring of transmission levels in areas that have achieved elimination targets. Platforms that allow testing in basic laboratories or testing of easy-to-manage samples such as dried blood spots would contribute to the feasibility of serologic testing. Blood from 506 1-12-year-olds in 2 villages in Kongwa district, Tanzania, was tested for antibodies against the antigen Pgp3. Whole blood, plasma, and dried blood spots (DBS) were tested in lab and field settings using a cassette-enclosed Pgp3 lateral flow assay (LFA-cassette) and a pared-back "dipstick" assay (LFA-dipstick). DBS were also tested with a bead-based multiplex assay (MBA). There was no significant difference in antibody positivity between the MBA and either LFA format (ranging from 42.5% to 48.4%). Interrater agreement between an expert rater and 3 different raters in field and lab settings was uniformly good, with Cohen's kappa >0.81 in all cases. |
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