Last data update: Apr 29, 2024. (Total: 46658 publications since 2009)
Records 1-2 (of 2 Records) |
Query Trace: Pendergrast EC [original query] |
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An HPLC ultraviolet method using low sample volume and protein precipitation for the measurement of retinol in human serum suitable for laboratories in low- and middle-income countries
Chaudhary-Webb M , Schleicher RL , Erhardt JG , Pendergrast EC , Pfeiffer CM . J Appl Lab Med 2019 4 (1) 101-107 BACKGROUND: Assessing vitamin A status in populations remains a high public health priority for low- and middle-income countries. However, analytical difficulties with serum retinol measurements persist in international laboratories. Nearly all participants in a Centers for Disease Control and Prevention external quality assessment program use HPLC to measure serum retinol, but round-to-round results failing to meet acceptable criteria suggest the need to provide a straightforward stable HPLC ultraviolet (UV) method that can be adopted by these laboratories to improve performance. We present a protein precipitation HPLC-UV method that measures serum retinol below the deficiency cutoff value (<0.7 mumol/L or 20 mug/dL) that is suitable for low- and middle-income countries and uses commercially available materials. METHODS: Serum (25 muL) added to retinyl acetate was precipitated with acetonitrile (125 muL) to extract retinol. Solvent-based calibration solutions required no extraction. Calibration used either single-point (50 mug/dL) or multipoint solutions (0.52-100 mug/dL). C18 column (4.6 x 100 mm) and acetonitrile with 0.1% triethylamine/water (83/17, v/v) as isocratic mobile phase (1.1 mL/min), achieved baseline separation (7 minutes). RESULTS: With only 25 muL of serum, the limit of detection was 0.52 mug/dL. Single- and multipoint calibration generated equivalent results. Over several years, between-run imprecision was </=7.1% in multiple quality-control materials. Overall mean (CV) method bias for NIST-certified reference materials (e-series) was -0.2% (5.8%). Maximally, 180 samples were processed within 24 h. CONCLUSIONS: This method was robust and stable over years and accurately measured serum retinol with low-volume samples. Thus, it may be of interest to low- and middle-income countries and to pediatric and finger stick applications. |
Development of a Standard Reference Material for metabolomics research
Phinney KW , Ballihaut G , Bedner M , Benford BS , Camara JE , Christopher SJ , Davis WC , Dodder NG , Eppe G , Lang BE , Long SE , Lowenthal MS , McGaw EA , Murphy KE , Nelson BC , Prendergast JL , Reiner JL , Rimmer CA , Sander LC , Schantz MM , Sharpless KE , Sniegoski LT , Tai SS , Thomas JB , Vetter TW , Welch MJ , Wise SA , Wood LJ , Guthrie WF , Hagwood CR , Leigh SD , Yen JH , Zhang NF , Chaudhary-Webb M , Chen H , Fazili Z , Lavoie DJ , McCoy LF , Momin SS , Paladugula N , Pendergrast EC , Pfeiffer CM , Powers CD , Rabinowitz D , Rybak ME , Schleicher RL , Toombs BM , Xu M , Zhang M , Castle AL . Anal Chem 2013 85 (24) 11732-8 The National Institute of Standards and Technology (NIST), in collaboration with the National Institutes of Health (NIH), has developed a Standard Reference Material (SRM) to support technology development in metabolomics research. SRM 1950 Metabolites in Human Plasma is intended to have metabolite concentrations that are representative of those found in adult human plasma. The plasma used in the preparation of SRM 1950 was collected from both male and female donors, and donor ethnicity targets were selected based upon the ethnic makeup of the U.S. population. Metabolomics research is diverse in terms of both instrumentation and scientific goals. This SRM was designed to apply broadly to the field, not toward specific applications. Therefore, concentrations of approximately 100 analytes, including amino acids, fatty acids, trace elements, vitamins, hormones, selenoproteins, clinical markers, and perfluorinated compounds (PFCs), were determined. Value assignment measurements were performed by NIST and the Centers for Disease Control and Prevention (CDC). SRM 1950 is the first reference material developed specifically for metabolomics research. |
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