Last data update: Dec 02, 2024. (Total: 48272 publications since 2009)
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Query Trace: Jung KY[original query] |
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An improved high-performance liquid chromatography-tandem mass spectrometric method to measure atrazine and its metabolites in human urine
Panuwet P , Restrepo PA , Magsumbol M , Jung KY , Montesano MA , Needham LL , Barr DB . J Chromatogr B Analyt Technol Biomed Life Sci 2010 878 957-62 We report an improved solid-phase extraction-high-performance liquid chromatography-tandem mass spectrometry method with isotope dilution quantification to measure seven atrazine metabolites in urine. The metabolites measured were hydroxyatrazine (HA), diaminochloroatrazine (DACT), desisopropylatrazine (DIA), desethylatrazine (DEA), desethylatrazine mercapturate (DEAM), atrazine mercapturate (ATZM), and atrazine (ATZ). Using offline mixed-mode reversed-phase/cation-exchange solid-phase extraction dramatically increased recovery and sensitivity by reducing the influence of matrix components during separation and analysis. DACT extraction recovery improved to greater than 80% while the other analytes had similar extraction efficiencies as previously observed. Limits of detection were lower than our previous method (0.05-0.19ng/mL) with relative standard deviations less than 10%. The total runtime was shorter (18min) than the previous on-line method, thus it is suitable for large-scale sample analyses. We increased the throughput of our method twofold by using the newer extraction technique. |
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