Last data update: Nov 04, 2024. (Total: 48056 publications since 2009)
Records 1-2 (of 2 Records) |
Query Trace: Humphrey CD[original query] |
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Aggregation of adenovirus 2 in source water and impacts on disinfection by chlorine
Kahler AM , Cromeans TL , Metcalfe MG , Humphrey CD , Hill VR . Food Environ Virol 2016 8 (2) 148-55 It is generally accepted that viral particles in source water are likely to be found as aggregates attached to other particles. For this reason, it is important to investigate the disinfection efficacy of chlorine on aggregated viruses. A method to produce adenovirus particle aggregation was developed for this study. Negative stain electron microscopy was used to measure aggregation before and after addition of virus particles to surface water at different pH and specific conductance levels. The impact of aggregation on the efficacy of chlorine disinfection was also examined. Disinfection experiments with human adenovirus 2 (HAdV2) in source water were conducted using 0.2 mg/L free chlorine at 5 degrees C. Aggregation of HAdV2 in source water (≥3 aggregated particles) remained higher at higher specific conductance and pH levels. However, aggregation was highly variable, with the percentage of particles present in aggregates ranging from 43 to 71 %. Upon addition into source water, the aggregation percentage dropped dramatically. On average, chlorination CT values (chlorine concentration in mg/L x time in min) for 3-log10 inactivation of aggregated HAdV2 were up to three times higher than those for dispersed HAdV2, indicating that aggregation reduced the disinfection rate. This information can be used by water utilities and regulators to guide decision making regarding disinfection of viruses in water. |
First detection of group C rotavirus in children with acute gastroenteritis in South Korea.
Moon S , Humphrey CD , Kim JS , Baek LJ , Song JW , Song KJ , Jiang B . Clin Microbiol Infect 2010 17 (2) 244-7 Group C rotavirus (GpC RV) causes sporadic cases and outbreaks of acute diarrhea in humans worldwide, but has not been detected among children in South Korea. The aims of the present study were to detect GpC RV among children hospitalized with gastroenteritis in South Korea and perform molecular characterization of GpC RV strains. From November 2003 to January 2006, 434 fecal samples were collected from children < 10 years of age who were hospitalized for treatment of acute diarrhea and screened for group C and A rotaviruses by EIA. GpC RV strains were characterized by sequence and phylogenetic analysis.Of the 434 samples screened, two were positive for GpC RV and one had a mixed GpC and GpA RV infection. One of the strains, Icheon, shared high sequence conservation in VP4, VP6 and VP7 genes with other published GpC RV. This is the first report describing the molecular characteristics of GpC RV among children in South Korea, more surveillance is needed to determine the burden of GpC RV diarrhea. |
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