OBJECTIVES: To describe the characteristics of cases of Ebola virus disease (EVD) nosocomial infections (NIs) in the Democratic Republic of the Congo, July 2018-May 2020, to inform future interventions. METHODS: We identified cases of NI during EVD outbreak response surveillance, and conducted a retrospective analysis of cases according to demographic characteristics and health facility (HF) type. RESULTS: Of 3481 cases of EVD, 579 (16.6%) were NIs, 332 of which occurred in women (57.3%). Patients and visitors accounted for 419 cases (72.4%), of which 79 (18.9%) were aged from 6 to ≤ 18 years and 108 (25.8%) were aged ≤ 5 years. Health workers (HWs) accounted for the remaining 160 (27.6%) NI cases. Case fatality rate (CFR) among HWs (66/160; 41.3%) was significantly lower than among patients and visitors (292/419; 69.7%) (p < 0.001). CFR was higher among those aged 6-18 years (54/79; 68.4%) and ≤ 5 years (89/108; 82.4%). Referral HFs (> 39 beds) had the highest prevalence of EVD NI (148/579; 25.6%). Among HFs with at least one case of NI, 50.0% (98/196) were privately owned. CONCLUSIONS: nurses and traditional healers should be targeted for IPC training, and supportive supervision provided to HFs to mitigate EVD transmission.