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COVID-10 GPH

Last data update: Mar 21, 2025. (Total: 48935 publications since 2009)
(Author names in bold with hyperlink are CDC authors)
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Records 1-10 (of 10 Records)
Query Trace: Garcia-Toledo L[original query]
PacBio Genome Sequences of Escherichia coli Serotype O157:H7, Diffusely Adherent E. coli , and Salmonella enterica Strains, All Carrying Plasmids with an mcr-1 Resistance Gene.
Lindsey RL , Batra D , Smith P , Patel PN , Tagg KA , Garcia-Toledo L , Loparev VN , Juieng P , Sheth M , Joung YJ , Rowe LA .
Microbiol Resour Announc 2018 7 (14) type type

We report here Illumina-corrected PacBio whole-genome sequences of an Escherichia coli serotype O157:H7 strain (2017C-4109), an E. coli serotype O[undetermined]:H2 strain (2017C-4173W12), and a Salmonella enterica subsp. enterica serovar Enteritidis strain (2017K-0021), all of which carried the mcr-1 resistance gene on an IncI2 or IncX4 plasmid. We also determined that pMCR-1-CTSe is identical to a previously published plasmid, pMCR-1-CT.
High-Quality Whole-Genome Sequences for 77 Shiga Toxin-Producing Escherichia coli Strains Generated with PacBio Sequencing.
Patel PN , Lindsey RL , Garcia-Toledo L , Rowe LA , Batra D , Whitley SW , Drapeau D , Stoneburg D , Martin H , Juieng P , Loparev VN , Strockbine N .
Genome Announc 2018 6 (19) type type

Shiga toxin-producing Escherichia coli (STEC) is an enteric foodborne pathogen that can cause mild to severe illness. Here, we report the availability of high-quality whole-genome sequences for 77 STEC strains generated using the PacBio sequencing platform.
High-Quality Whole-Genome Sequences for 59 Historical Shigella Strains Generated with PacBio Sequencing.
Kim J , Lindsey RL , Garcia-Toledo L , Loparev VN , Rowe LA , Batra D , Juieng P , Stoneburg D , Martin H , Knipe K , Smith P , Strockbine N .
Genome Announc 2018 6 (15) type type

Shigella spp. are enteric pathogens that cause shigellosis. We report here the high-quality whole-genome sequences of 59 historical Shigella strains that represent the four species and a variety of serotypes.
High-Quality Complete and Draft Genome Sequences for Three Escherichia spp. and Three Shigella spp. Generated with Pacific Biosciences and Illumina Sequencing and Optical Mapping.
Schroeder MR , Juieng P , Batra D , Knipe K , Rowe LA , Sheth M , Smith P , Garcia-Toledo L , Loparev VN , Lindsey RL .
Genome Announc 2018 6 (1) type type

Escherichia spp., including E. albertii and E. coli, Shigella dysenteriae, and S. flexneri are causative agents of foodborne disease. We report here reference-level whole-genome sequences of E. albertii (2014C-4356), E. coli (2011C-4315 and 2012C-4431), S. dysenteriae (BU53M1), and S. flexneri (94-3007 and 71-2783).
High-Quality Whole-Genome Sequences for 21 Enterotoxigenic Escherichia coli Strains Generated with PacBio Sequencing.
Smith P , Lindsey RL , Rowe LA , Batra D , Stripling D , Garcia-Toledo L , Drapeau D , Knipe K , Strockbine N .
Genome Announc 2018 6 (2) type type

Enterotoxigenic Escherichia coli (ETEC) is an important diarrheagenic pathogen. We report here the high-quality whole-genome sequences of 21 ETEC strains isolated from patients in the United States, international diarrheal surveillance studies, and cruise ship outbreaks.
High-Quality Draft Genome Sequences for Four Drug-Resistant or Outbreak-Associated Shigella sonnei Strains Generated with PacBio Sequencing and Whole-Genome Maps.
Lindsey RL , Batra D , Rowe L , Loparev V N , Juieng P , Garcia-Toledo L , Bicknese A , Stripling D , Martin H , Chen J , Strockbine N , Trees E .
Genome Announc 2017 5 (35) type

Drug-resistant Shigella sonnei poses a clinical and public health challenge. We report here the high-quality draft whole-genome sequences of four outbreak-associated S. sonnei isolates; three were resistant to two or more antibiotics, and one was resistant to streptomycin only.
Multiplex polymerase chain reaction for identification of Escherichia coli, Escherichia albertii and Escherichia fergusonii.
Lindsey RL , Garcia-Toledo L , Fasulo D , Gladney LM , Strockbine N .
J Microbiol Methods 2017 140 1-4 type

Escherichia coli, Escherichia albertii, and Escherichia fergusonii are closely related bacteria that can cause illness in humans, such as bacteremia, urinary tract infections and diarrhea. Current identification strategies for these three species vary in complexity and typically rely on the use of multiple phenotypic and genetic tests. To facilitate their rapid identification, we developed a multiplex PCR assay targeting conserved, species-specific genes. We used the Daydreamer (Pattern Genomics, USA) software platform to concurrently analyze whole genome sequence assemblies (WGS) from 150 Enterobacteriaceae genomes (107 E. coli, 5 Shigella spp., 21 E. albertii, 12 E. fergusonii and 5 other species) and design primers for the following species-specific regions: a 212bp region of the cyclic di-GMP regulator gene (cdgR, AW869_22935 from genome K-12 MG1655, CP014225) for E. coli/Shigella; a 393bp region of the DNA-binding transcriptional activator of cysteine biosynthesis gene (EAKF1_ch4033 from genome KF1, CP007025) for E. albertii; and a 575bp region of the palmitoleoyl-acyl carrier protein (ACP)-dependent acyltransferase (EFER_0790 from genome ATCC 35469, CU928158) for E. fergusonii. We incorporated the species-specific primers into a conventional multiplex PCR assay and assessed its performance with a collection of 97 Enterobacteriaceae strains. The assay was 100% sensitive and specific for detecting the expected species and offers a quick and accurate strategy for identifying E. coli, E. albertii, and E. fergusonii in either a single reaction or by in silico PCR with sequence assemblies.
High-Quality Genome Sequence of an Escherichia coli O157 Strain Carrying an mcr-1 Resistance Gene Isolated from a Patient in the United States.
Lindsey RL , Batra D , Rowe L , Loparev VN , Stripling D , Garcia-Toledo L , Knipe K , Juieng P , Sheth M , Martin H , Laufer Halpin A .
Genome Announc 2017 5 (11) type

Enterobacteriaceae carrying plasmid-mediated colistin resistance have been found around the world. We report here the high-quality whole-genome sequence of an Escherichia coli O157:H48 isolate (2016C-3936C1) from Connecticut that carried the mcr-1 resistance gene on an IncX4-type plasmid.
High-Quality Draft Genome Sequences for Five Non-O157 Shiga Toxin-Producing Escherichia coli Strains Generated with PacBio Sequencing and Optical Maps.
Lindsey RL , Rowe L , Garcia-Toledo L , Loparev V , Knipe K , Stripling D , Martin H , Trees E , Juieng P , Batra D , Strockbine N .
Genome Announc 2016 4 (3) type

Shiga toxin-producing Escherichia coli (STEC) is a foodborne pathogen. We report here the high-quality draft whole-genome sequences of five STEC strains isolated from clinical cases in the United States. This report is for STEC of serotypes O55:H7, O79:H7, O91:H14, O153:H2, and O156:H25.
Complete Genome Sequences of Two Shiga Toxin-Producing Escherichia coli Strains from Serotypes O119:H4 and O165:H25.
Lindsey RL , Knipe K , Rowe L , Garcia-Toledo L , Loparev V , Juieng P , Trees E , Strockbine N , Stripling D , Gerner-Smidt P .
Genome Announc 2015 3 (6) type

Shiga toxin-producing Escherichia coli (STEC) is an important foodborne pathogen. Here, we report complete whole-genome sequences for two STEC strains of serotypes O119:H4 and O165:H25 isolated from clinical cases in the United States.
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